Venture Chemical Ltd. 652, Fukano, Muro, Uda City, NARA 632-0203 JAPAN Telephone : +81-745-97-2111 Facsimile : +81-745-97-2112 http://www.venture-chemical.co.jp “IOCA-C21” - unique fibre synthetised with negatively-ionized fine particles and carbon atoms by applying nanotechnology offers physiological and biomedical functions 　Two main physical and biomedical factors for the homeostasis maintenance in human body are negative irons and far-infrared radiation (FIR) which may provide hormetic and diathermic effect respectively. These hormetic and diathermic effects are clinically beneficial for various functions in the living body. Namely, they may encourage metabolic functions and vitalize weakened immune functions in human body. 　The main components of the functional fibre of IOCA-C21 may produce negative ions and FIR originated and induced from ore (tourmaline) and Bincho-Charcoal which have grained down to corpuscle (nanometric size) by using the nanotechnological procedure. 　IOCA-C21 fibre, emitting negative ions and FIR, may work on metabolic functions of living body and activate immune functions. For instance, it can enhance the non-specific immune function in the immunological surveillance system that activates macrophages and natural killer (NK) cells of immunocompetent cells. 　IOCA-C21 fibre is clinically useful for human body having sensitive skin and/or several skin disorders. The epoch-making material may constantly give the both negative ions and IFR to skin, so that the skin may be revived with moisture and shine. Microscopic image of IOCA-C21: particles of Bincho-Charcoal are visible in spots Three Major Biomedical Functions of IOCA-C21 ■ Immune system activation IOCA-C21 fibre enhances the non-specific immune function which is one of the immunological surveillance systems, where especially activates macrophage and NK cell functions in immunocompent cells. Therefore, it can be commendable to human having sensitive skin and/or skin disorders. Feasibility data from human subject research Illustration A) IOCA-C21 knee supporters were worn by a 9-year-old boy who had atopic dermatitis. Picture on the left showed the back of knee before wearing supporters, and picture on the right showed the same 3 days after wearing them. Black spots almost disappeared, and the boy stopped scratching which he previously did unconsciously. Illustration B) IOCA-C21 pajama was worn by a 12-year-old boy who had chronic skin disorder. The following pictures shows change in appearance during 7 days before and after wearing the pajama. Red spots and scratch were decreased by wearing the pajama for 7 days. Probably the boy didn't scratch while sleeping. He was happy with its soft touch. Before 3 days later 7 days later Illustration C) The effect of IOCA-C21 cloth on human immune system was determined in terms of number of monocytes, lymphocytes and leukocytes by 10 medical students (5 males and 5 females) of Gifu University. On the first day of clinical test, subjects spent a normal time with normal clothing, and their blood was collected at 14:00 to be tested. On the second day, their blood was again collected at 14:00 after they spent a normal time. Then their cloth was changed in IOCA-C21 cloth. On the third day, their blood was again collected at 14:00 after they spent a normal time in IOCA-C21 cloth. The result of clinical test: As the result of clinical test with 10 healthy subjects during those 3 days, number of monocytes was increased 1.3 % on average. This suggests the possibility of IOCA-C21 cloth to activate human immune system. Microscopic image of red blood cells [Before using IOCA-C21 cloth] Sticky [After used IOCA-C21 cloth] Smooth Feasibility data from animal subject research The effect of IOCA-C21 fibre on wounded subcutaneous tissue of mouse was observed through microscopic images. 1.　Normal subcutaneous tissue 2.　Wounded subcutaneous tissue 3.　Recovered subcutaneous tissue 14 days after applied IOCA-C21 fibre (Details of the clinical test by using mouse should be disclosed upon request) ■ Blood circulation promotion through diathermic effect IOCA-C21 emits Far-infrared radiation, a solar ray with wavelength between 4 and 22 microns which is called “rearing light”, being essential for the growth of living things to produce diathermic effect. An optimum range of the rearing light for human body is with wavelength between 5 and 10 microns, reaching deeper parts of the body to convert into thermal energy. The body is warmed from inside, which then promotes vasodilatation, blood flow increase, and blood circulation. Consequently, blood circulative disorders such as hypertension and arteriosclerosis may be prevented. Moreover, body cells may be activated by fresh oxygen fed through stimulated blood circulation to reproduce ATP energy in the body. Far-infrared radiation is an essential solar ray for living things without side effect. Test data No. 1 Thermal property of IOCA-C21 was evaluated by heating samples with IR radiation and measuring the degree of heat storage and release by a thermographer. Samples: 　　(A) 100 % IOCA-C21 cloth (B) 50 % IOCA-C21 cloth (C) Control Procedure: Samples were placed on a flat table to be exposed to IR radiation from a height of 60 cm. The change of surface temperature by heating and heat release after stopped heating were determined via thermographic image. Areas of each sample were framed with white line to compare them easily in accordance with colour bar on the right side which indicates average, minimum and maximum temperature of sample (A) (B) and (C). Result: Average temperature increase by (A) 100% IOCA-C21 and (B) 50% IOCA-C21 was 5.8℃ and 4.3℃ respectively in terms of heat storage by 60 seconds after exposure to IR radiation. On the other hands, the same by (C) control was 2.1℃. It suggests the superiority of IOCA-C21 cloth in terms of heat storage and release. 　　 (A) 100% IOCA-C21 (B) 50% IOCA-C21 (C) Control　 Before exposure　　 AV. 26.7 ℃ 26.5 ℃ 26.5 ℃ MIN. 26.5 ℃ 26.3 ℃ 26.3 ℃ MAX. 26.9 ℃ 26.7 ℃ 26.8 ℃ 60 sec. after exposure AV. 32.5 ℃ 30.8 ℃ 28.6 ℃ MIN. 31.3 ℃ 30.0 ℃ 28.2 ℃ MAX. 33.2 ℃ 31.6 ℃ 29.1 ℃ 10 sec. after heat release AV. 30.1 ℃ 29.1 ℃ 27.8 ℃ MIN. 29.3 ℃ 28.3 ℃ 27.5 ℃ MAX. 30.8 ℃ 29.6 ℃ 28.3 ℃ Test data No. 2 Variation in blood volume, flow velocity and flow rate in case of wearing IOCA-C21 cloth were determined. ■ Metabolism promotion through homeostatic effect Human body is composed of about 60 % water including bodily fluid and water molecules cluster of blood. Negative ions emitted by IOCA-C21 may be absorbed by human body to transform the body water to mist-like form (of which phenomenon is called “Lenard effect”). Negatively ionized body water (bodily fluid, blood, lymph fluid, etc.) is circulated in the body to deacidify the whole body water, which may normalize blood circulation, blood viscosity, blood flow, etc. This blood normalization may be accelerated by synergic effects of negative ions and far-infrared radiation. Good blood circulation activates metabolic functions as well as division and physiology of cells (epidermal cells, fibroblast, immunological surveillance system, etc.). As a result, three major body systems (including nerve system, internal secretion system, and immune system) may be activated to maintain homeostasis and healthy body with resistance to diseases. Test data Number of negative ions in the presence or absence of 100 % IOCA-C21 cloth in room was determined by Ecoholistic's ion counter EB-12A with ambient temperature of 24.6℃ and humidity of 56%. Average ions in the presence of 100 % IOCA-C21 cloth was 1510 ions/cc, while the same in the absence was 43 ions per/cc. Incidental Functions of IOCA-C21 ■ Antibacterial and odour killing efficacy Antibacterial and odour killing efficacy are synergic effect of ore and Bicho-Chacoal contained in IOCA-C21. Test data No. 1 IOCA-C21 cloth exhibited antibacterial efficacy as the result of antibacterial test carried out according to JIS L 1902. [Test method] Quantitative test according to JIS L 1902 Number of active bacteria was determined according to pour plate method [Species of bacteria] Staphylococcus aureus 　ATCC 6538P [Test result] Number of inoculated bacteria [A] 2.1 × 104 log A 4.3 Number of bacteria on blank(*) [B] 1.3 × 107 log B 7.1 (*) Blank: regular cotton cloth log B - log A = 2.8 Bactericidal activity = log A - log C Bacteriostatic activity = log B - log C Sample Log C Bactericidal activity Bacteriostatic activity Standard value IOCA-C21 cloth 2.7 1.6 4.4 > 1.5 (Tested by Japan Spinners Inspecting Foundation) Test data No. 2 IOCA-C21 cloth exhibited odour killing property as the result of gas adsorption test conducted by Japan Synthetic Textile Inspection Institute Foundation as follows: [Test method] 15 g sample was put in 5-liter gas sampling bag (Tedlar® bag) and 3 liters gas (*) with specified concentration was injected. Then, gas concentration was determined with detecting tube 2 and 24 hours later. (*) ammonia (NO.3L), acetic acid (NO.81), hydrogen sulfide (NO.4LL) Sample　 Ammonia (ppm)　　 Initial 2 hours later 24 hours later IOCA-C21 cloth 20 < 0.5 < 0.5 Blank 20 17 12 Sample　 Acetic acid (ppm)　　 Initial 2 hours later 24 hours later IOCA-C21 cloth 20 < 1.0 < 1.0 Blank 20 10 5.0 Sample　 Hydrogen sulfide (ppm)　　 Initial 2 hours later 24 hours later IOCA-C21 cloth 15 13 6.5 Blank 15 15 14 ■ Possible anticancer effect It has been discovered that some nameless species of bacteria exist in Bicho-Chacoal as shown in the following microscopic images dyed with phenosafranine (10 × 100 = 1000). (investigated by the national institute of advanced industrial science and technology, Japan) Experimentation to check any of effect of these bacteria on sarcoma was carried out. The following pictures show a series of sarcomas in original state (upper) and the same 28 days after these bacteria were injected to them (lower). Shrunk sarcomas may suggest possible anticancer effect of these bacteria. (bar = 20 mm) Safety Data of IOCA-C21 ■ Skin patch test data Closed patch test was carried out with 20 human subjects by Life Science Laboratory Ltd., and no primary irritation after 24 hours was found for all 20 subjects. Practical Use of IOCA-C21 ■ Athletic supporter ■ Underwear ■ Athlete's apparel ASICS provided Netherland's national team uniforms for Torino Olympic game. Textile products of IOCA-C21 are approved and recommended by Japan Atopic Dermatitis Patients Association Japan Atopic Dermatitis Patients Association Certificate of Recommended Article Mark Recommended article: 　　　Unique fiber “IOCA-C21” Approval No. T 506500 A This mark indicates the article recommended by Japan Atopic Dermatitis Patients Association, The designation of recommended articles aims at life support for sufferers from atopic dermatitis and allergy disease as well as contribution to the improvement of treatment environment by distributing the right information about these diseases. Textile products of IOCA-C21 are approved and recommended by International Health Association The item approved by International Health Association (IHA) Approval No. P1015 Fibre, “IOCA-C21” used in this item has been approved by International Health Association (IHA). IHA aims at contribution to the improvement of health, welfare and quality of life for people, as well as enlightenment, guidance and prevention of not only lifestyle diseases and allergy diseases but also growing emotional disorder of children and youngster through medical approach and study. IHA's purpose of activity We have acquired convenient and hygiene life environment backed by the progress of civilization and the development of science technology, where many diseases have been treatable and average life expectancy has been extended. On the contrary, people living in some countries have been increasingly suffering civilization diseases such as lifestyle diseases or allergy diseases. Advanced countries including Japan began facing important issues of lifestyle diseases which have been expanded to youngsters and the increase in allergy diseases. Atopic dermatitis patients, for example, are suffered with the appearance of atopic skin symptoms which may lead to bullying (particularly among children) or to isolation from other people even among adults. Some of asthma patients are also isolated from normal social life regardless of their age. Under such backgrounds, new business has been created to prevent and treat such diseases as providing various articles and services. And the media has picked up information regarding diseases and health. Normally, consumers must be given a certain benefit by using information, articles and services. However, it is regretful that a few articles or services are full of commercialism and some media provide information just to catch onto health fad. We, incorporated nonprofit organization of International Health Association (IHA) shall investigate, study, illumine and solve these issues overall, and shall act for the purpose of contribution to the improvement of health, welfare and quality of life of people through establishing guidelines with respect to products and services to maintain and improve health. Moreover, we understand that truancy and withdrawal are modern problems involving children or youngsters who should create future society. And we came to know that it is necessary to tackle and solve such problems comprehensively with medical approach, although it is not yet developed sufficiently. In order to provide mental and physical health support to children and youngsters, we have established Heart Care Center that works on the improvement of quality of life of them including their family and affiliates. Outline of the organization Name of organization Incorporated nonprofit organization of International Health Association (IHA) Location of head office 251-2, Takeyano-cho, Shinogyo-ku, Kyoto 600-8090 Japan Contact Tel 　075-354-9919 Fax 　075-354-3299 Office representatives Sawako Takahashi Yuko Nishida Location of branch office 5-27, Hie-cho, Hakata-ku, Fukuoka, Japan Board of Administration [Emeritus administrators] Yukio Yanagimoto (MD) Former principle of Shitennoji International Buddhist University, Supreme advisor of Life Science Laboratories Ltd. Takeo Fujino (MD) Emeritus Professor of Kyushu University, Director of medical corporation “Kenjinkai” Muneo Yoshikawa (PhD) Emeritus Professor of Hawaii University, Principle of International Mentorship Graduate School 　 　 [Chief Administrator] Naoki Sai (DBA) Representative of Eternal Light Group [Administrators] Yoshinari Isobe (MD) Director of Isobe Clinic Junryo Onoda Director of Rainbow Therapy Akihiko Ishihara (Ph.D) Professor of Graduate School of Human and Environmental Studies, Kyoto University Hiroshi Kawashima (MD) Assistant Professor of Tokyo Women's Medical University, Director of Aoyama Woman Natural Medical Laboratory Taisei Kageyama (HSD) Director of Takawa Clinic, Director of Clinic Ginza Hiroaki Kumano (MD) Assistant Professor of Graduate School of Medicine and Faculty of Medicine, The University of Tokyo Koji Takagi (MD) Assistant of Graduate School Faculty of Medical Sciences, Kyushu University Shigeki Watanuki (Ph.D) Professor of Department of Physiological Anthropology Faculty of Design, Kyushu University Hiroshi Fukuoka (MD) Director of Medical Corporation “Meitokukai”, Director of Fukuoka Dental Clinic Noriyuki Tatsumi (MD) Emeritus Professor of Osaka University, Professor of Shitennoji International Buddhist University Akihiro Inanaga Branch Office Manager of IHA, President of Actbiz KK.  　 　 [Supervisors] Katsuaki Horoo Professor of Hosei University Faculty of Law Ryuhei Nishimura (CPA) Director of Kyoto Kitayama Intellectual Property Office (C) copyright 2011 Venture Chemical Ltd.All Right Reserved.